| 產(chǎn)品名稱 |
Enterobacter cloacae |
| 商品貨號(hào) |
B81052 |
| 中文名稱 |
陰溝腸桿菌 |
| 模式菌株 |
模式菌株 |
| 其它保藏中心編號(hào) |
CICC21539 =ATCC13047=NCDC279-56=IFO13535=NCTC10005 |
| 特征特性 |
Nucleotide (GenBank) : AX109551 Sequence 284 from Patent WO0123604. Nucleotide (GenBank) : AX111486 Sequence 2219 from Patent WO0123604. Nucleotide (GenBank) : AX111507 Sequence 2240 from Patent WO0123604. Nucleotide (GenBank) : AX111527 Sequence 2260 from Patent WO0123604. Nucleotide (GenBank) : AF052256 Enterobacter cloacae DNA gyrase A (gyrA) gene, partial cds. Nucleotide (GenBank) : AY013713 Enterobacter cloacae retro-nitroreductase gene, complete cds Nucleotide (GenBank) : U65720 Enterobacter cloacae 16S ribosomal RNA gene, partial sequence. Nucleotide (GenBank) : U77435 Enterobacter cloacae RNA polymerase beta-subunit (rpoB) gene, partial cds. Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980. API 20E QC Set. bioMerieux. . Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int. J. Syst. Evol. Microbiol. 55: 1395-1397, 2005. PubMed: 16014455 . Reassignment of Enterobacter dissolvens to Enterobacter cloacae as E. cloacae subspecies dissolvens comb. nov. and emended description of Enterobacter asburiae and Enterobacter kobei . Syst. Appl. Microbiol. 28: 196-205, 2005. PubMed: 15900966 ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions) Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM. API 10S QC Set. bioMerieux. |
| 具體用途 |
應(yīng)用于質(zhì)量控制應(yīng)變,API和易感效價(jià)質(zhì)量控制應(yīng)變 |
| 培養(yǎng)基 |
營養(yǎng)瓊脂或營養(yǎng)肉湯培養(yǎng)基 |
| 培養(yǎng)溫度 |
30 ℃, 有氧 |
| 分離基物 |
脊髓液 |
| 凍干物說明 |
·凍干首次活化,干粉要全部用完,不能保留,用0.2-0.5m1的培養(yǎng)液或者無菌水溶解,接種在1-2個(gè)平板上,因凍干菌種處于休眠狀態(tài),請(qǐng)勿接種多個(gè)平板,以免因接種量不足而導(dǎo)致復(fù)蘇不成功； |
| 保存說明 |
1、長期保存的儲(chǔ)存菌株以冷凍干燥保藏為主，特殊要求的菌種可用其它方法進(jìn)行保存，保存期可長達(dá)幾年至幾十年。2、半儲(chǔ)存菌株以含20%甘油的肉湯或液體石臘封存的半固體瓊脂為主，在0℃以下保存，保存期為3－6個(gè)月。3、應(yīng)用菌株以瓊脂斜面為主，在4℃保存，保存期為1－3個(gè)月。4、菌種的傳代不可超過6次。視菌種的特性及使用情況，一般儲(chǔ)存菌株每5年傳記代一次，每1-2年從儲(chǔ)存菌株轉(zhuǎn)接至半儲(chǔ)存菌株，半儲(chǔ)存菌株每3-6個(gè)月傳代一次，每1-3個(gè)月從半儲(chǔ)存菌株轉(zhuǎn)接接至應(yīng)用菌株。 |
