Store fetal bovine serum at -70°C. If storage at -70°C is not possible, then store at -20°C. Do not store fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
Applications
This serum is tested for ability to support the growth and maintenance of embryonic stem cells in an undifferentiated state. Undifferentiated embryonic stem cells are determined by colony morphology and expression of three pluripotent markers. The results are compared to a control lot of serum. The serum is triple filtered through 0.1µM filters and each lot of fetal bovine serum is tested for sterility. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA inspected abattoirs located in the United States.
Comments
The results were compared with cells grown with a control lot of serum.
Biosafety Level
N/A
Quality Control Specifications
Sterility tests are performed on each lot of fetal bovine serum using current USP and EP methods. Specific tests for contaminants are listed below in the Specification Certificate. This serum is tested for ability to support the growth and maintenance of embryonic stem cells in an undifferentiated state. Undifferentiated embryonic stem cells are determined by colony morphology and expression of three pluripotent markers. The results are compared to a control lot of serum. The serum is triple filtered through 0.1 μM filters and each lot of fetal bovine serum is tested for sterility.
Origin
This fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.
