ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following restriction sites (nt positions according to the published sequence): HpaI--1188; PstI--373; PvuII--789; ScaI--1111; XbaI--1585.
Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--3.0, 2.0; PstI--5.0; PvuII--2.7, 1.5, 0.8; EcoRI--uncut; XbaI--4.7, 0.2.
The BstNI site at nt 8 was filled in and a HindIII linker was added. The 3' end of the insert includes the poly(A) tail, the added GC tail, and the BamHI site engineered from the original pMG-5 vector.
