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Hepatitis A virus

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編號:B238987

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產品名稱	Hepatitis A virus
商品貨號	B238987
Classification	Picornaviridae, Hepatovirus
Agent	Hepatitis A virus
Strain	HM175/18f
Applications	Efficacy testing Viruscide testing Enteric Research
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	frozen 1 mL per vial
Storage Conditions	-70°C or colder
Comments	Strain demonstrates a rapid replication/cytopathic effect (RR/CPE+) phenotype in BS-C-1 cells, but retains antigenic characteristics of low cell culture passage hepatitis A virus. CPE more pronounced in FRhK4 cells.
Effect on Host	CPE, refractile rounding and degeneration
Recommended Host	FRhK4 (ATCC^® CRL-1688™)
Growth Conditions	Temperature: 35°C Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 6-7 days
Name of Depositor	SM Lemon
Source	Human fecal material passaged in marmosets. Virus isolated from marmoset liver in primary cells then passaged in BS-C-1 cells.
References	Lemon SM, et al. Antigenic and genetic variation in cytopathic hepatitis A virus variants arising during persistent infection: evidence for genetic recombination. J. Virol. 65: 2056-2065, 1991. PubMed: 1705995 Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02. Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.

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