| Vector Information |
Size (kb): 7.1700000762939450 DESCRIPTION OF VECTOR:
Intact vector size: 7.170
Type of vector: plasmid
Cloning sites: NotI SacI SmaI BamHI XbaI
Polylinker sites: NotI EcoRI SacI AvaI KpnI SmaI BamHI XbaI
Other unique sites: HindIII
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 860-879
MCS: NotI...XbaI, ->, 908-942
replicon: 2 micron/LEU2-d, 941-3794
marker(s): LEU2-d, ->, 2298-3392
marker(s): URA3, <-, 3874-4676
restriction site: HindIII, 4899-4899
promoter for in vitro transcription: T3, <-, 4909-4928
promoter: lac, <-, 4998-5026
replicon: pMB1, 5352-5352
marker(s): ampR, <-, 6110-6970 Vector: YEpFAT5 (plasmid) Promoters: Promoter for in vitro transcription T7 Marker(s):URA3,ampR,LEU2-d Construct size (kb): 7.170000076293945 Features: marker(s): LEU2-d
marker(s): URA3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron/LEU2-d
replicon: pMB1
MCS: NotI...XbaI
restriction site: HindIII |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--7.0; SmaI--7.0; EcoRI--4.4, 2.0, 0.8. M9 + leucine + tryptophan medium recommended for DNA purification. Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200. One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine. |
