| 產品名稱 |
PRL-Rs3 probe |
| 商品貨號 |
B238053 |
| Designations |
PRL-Rs3 probe |
| GenBank Number |
M22957
|
| Species |
Mus musculus, mouse |
| Depositors |
DI Linzer |
| Vector |
Construct size (kb): 3.09500002861023 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.2259999960660934 Tissue: liver (pregnant) Gene product: prolactin receptor [Prlr] |
| Insert Size (kb) |
0.226 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): AvaII--2.8, 0.25; BamHI--3.1; BglI--3.1; DraI--2.4, 0.74; EcoRI--2.8, 0.28; PstI--3.1. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.03, HaeIII--0.15. A unique sequence probe corresponding to nt 1066-1291 of the published sequence for form 3 of the receptor. Transcription from the SP6 promoter gives the sense strand (linearize with HindIII. Transcription from the T7 promoter gives the anisense strand (linearze with BamHI). |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Clarke DL, Linzer DI. Changes in prolactin receptor expression during pregnancy in the mouse ovary. Endocrinology 133: 224-232, 1993. PubMed: 8319571
Davis JA, Linzer DI. Expression of multiple forms of the liver prolactin receptor in mouse liver. Mol. Endocrinol. 3: 674-680, 1989. PubMed: 2725531
Daniel I Linzer, personal communication
|
