| Vector |
Construct size (kb): 10.80000019073486 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC8
Intact vector size: 2.700
Type of vector: plasmid
Vector end: BamHI
Vector end: PstI
Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Construction: pBR322, Escherichia coli lacZalpha, M13mp8
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus thuringiensis subsp. kurstaki
Gene name: crystal protein (delta endotoxin)
Contains complete coding sequence?: Y
Strain: HD-1
Type of DNA: plasmid
Insert 5' end: BamHI
Insert 3' end: PstI
Insert size (kb): 8.1
Cross references: DNA Seq. Acc.: D00117 Insert lengths(kb): 8.100000381469727 Gene product: crystal protein (delta endotoxin) Target Gene: crystal protein (delta endotoxin) |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/PstI--8.1, 2.7; EcoRI--5.2, 1.9 (doublet), 0.7, 0.6; HindIII--8.0, 1.5, 1.1. The following microorganisms are suitable for recombination and plant-colonization: Pseudomonas, Agrobacterium, Rhizobium, Erwinia, Azotobacter, Azospirillum, Klebsiella, Alcaligenes and Flavobacterium. The coding sequence within the insert can be integrated into the chromosome of one of several plant-colonizing microorganisms to protect the colonized plant against insect pests. The complete coding sequence for the toxin is contained within an internal 4.6 kb HpaI-PstI fragment. |
| References |
Obukowicz MG, et al. Combatting plant insect pests with plant-colonizing microorganisms containing the toxin gene B. thuringiensis as a chromosomal insertion. US Patent 5,229,112 dated Jul 20 1993
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