| 產品名稱 |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| 商品貨號 |
B237870 |
| Deposited As |
Saccharomyces cerevisiae Hansen, teleomorph |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations |
XD99 |
| Alternate State |
Candida robusta Diddens et Lodder |
| Application |
At the same time the strain will produce red colonies instead of white and become Ade-, His-, Lys-, Ura- and Ilv-; ade2-1, his5-2, lys1-1, ura2-1 and ilv3-1 are also ochre-suppressible. |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Type Strain |
no |
| Genotype |
MATa/MATalpha ade2-1/ade2-1 his5-2/his5-2 lys1-1/lys1-1 can1-100/can1-100 +/ura2-1 SUP7/+ +/ilv3-1 SUP4/+ trp1/+
(M. Dixon, personal communication) |
| Preceptrol® |
no |
| Ploidy |
diploid |
| Comments |
Strains for detection of mutations, recombination, and aneuploidy (BG1) XD99 was constructed to screen for mitotic chromosome loss events. can1-100 is an ochre-suppressible mutation for canavanine resistance. As long as this strain carries both SUP4 and SUP7, it will be canavanine-sensitive. If it loses the copy of chromosome X that carries these two ochre suppressors, it will become canavanine-resistant. At the same time the strain will produce red colonies instead of white and become Ade-, His-, Lys-, Ura- and Ilv-; ade2-1, his5-2, lys1-1, ura2-1 and ilv3-1 are also ochre-suppressible. |
| Medium |
ATCC® Medium 1069: YPAD medium
|
| Growth Conditions |
Temperature: 25.0°C Atmosphere: Typical aerobic |
| Name of Depositor |
YGSC |
| Special Collection |
Yeast Genetic Stock Center |
| Chain of Custody |
ATCC <<--YGSC<<--M. Dixon |
| Isolation |
XD99 was constructed to screen for mitotic chromosome loss events. can1-100 is an ochre-suppressible mutation for canavanine resistance. As long as this strain carries both SUP4 and SUP7, it will be canavanine-sensitive. If it loses the copy of chromosome X that carries these two ochre suppressors, it will become canavanine-resistant. At the same time the strain will produce red colonies instead of white and become Ade-, His-, Lys-, Ura- and Ilv-; ade2-1, his5-2, lys1-1, ura2-1 and ilv3-1 are also ochre-suppressible. |
| References |
M. Dixon, personal communication
M. Dixon, personal communication
|
