Restriction digests of the clone give the following sizes (kb): EcoRI--4.8, 4.5; HindIII--9.3; PstI--6.2, 3.0; SacI--8.6, 0.52; XbaI--9.3. The insert contains the following restriction sites (approximate kb from the 5' end: SmaI--1.8, 3.4; SacI--2.1, 2.6; PstI--2.4; NcoI--2.8, XbaI--4.1. Contains the promoter region, exon 1 and part of intron 1. Subcloned from lambdaGHGR-5. ATCC 80008/9 and ATCC 80010/11 are contiguous in the genome. |
