Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.9, 0.24; XhoI--3.9, 0.96, 0.24; SmaI--4.7, 0.48; PvuII--2.5 (doublet). The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.05; SmaI--0.45; EcoRV--1.0; XhoI--1.02, 1.98; NcoI--1.6; EcoRI--1.95. Contains the complete coding sequence, with 8 nt of the 5' untranslated region and the complete 3' untranslated region. The 1.95 kb EcoRI fragment detects RNA of 2.5 kb in rat liver and rat skin (major) and 7.3 and 4.4 (minor). The 5' end of the insert is adjacent to the T7 promoter of the vector. |
