| Vector |
Construct size (kb): 8.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-3Zf(+)
Intact vector size: 3.199
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII
PstI SphI HindIII-SP6
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CEBPA
Gene name: CCAAT/enhancer binding protein (C/EBP), alpha
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.1
Tissue: umbilical vein endothelial cells
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 5.0
Cross references: DNA Seq. Acc.: U34070
Nucleotides ~700-4000 of the insert correspond to
nucleotides 1-3318 of U34070. Insert lengths(kb): 5.0 Tissue: umbilical vein endothelial cells Gene product: CCAAT/enhancer binding protein (C/EBP), alpha [CEBPA] Target Gene: CCAAT/enhancer binding protein (C/EBP), alpha |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.0, 3.2; HindIII--6.2, 1.5, 0.46; EcoRI--5.6, 2.5. Insert contains the following restriction sites (approximate kb from the 5' end): BglII--3.0; EcoRI--2.5; EcoRV--3.2; HindIII--3.1, 4.6; NcoI--1.3, 2.3; NotI--1.65, 1.7; PstI--0.7, 1.3, 1.85, 1.9, 4.4; SacI--2.3, 3.9; XbaI--3.5; XhoI--0.2, 2.35. The probe used for Northern blotting was the 0.7 kb EcoRI/HindIII fragment from the 3' untranslated region of the insert. |
| References |
Antonson P, Xanthopoulos KG. Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha). Biochem. Biophys. Res. Commun. 215: 106-113, 1995. PubMed: 7575576
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