| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.65, 2.8, 2.8; HindIII--3.85, 3.85, 2.9, 0.86, 0.45; PstI--5.7, 3.1, 2.3, 1.6; BamHI--6.6, 3.0, 2.7, indicating two vectors + insert. Codominant segregation demonstrated in 55 3-generation families for the EcoRI RFLP. The probe was pre-associated with total human DNA. Otherwise, RFLPs were observed under normal hybridization and wash stringencies Enzyme(s) not detecting polymorphism: MspI, TaqI, RsaI, BglII, PstI, PvuII, BamHI, HindIII. |
| References |
Hall JM, et al. Oncogenes and human breast cancer. Am. J. Hum. Genet. 44: 577-584, 1989. PubMed: 2564734
Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294
Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence for NRAS pMCR3 on chromosome 1. Nucleic Acids Res. 15: 9623, 1987. PubMed: 2891121
|
