| 產品名稱 |
pAR1151 |
| 商品貨號 |
B236406 |
| Designations |
pAR1151 |
| GenBank Number |
J02518
|
| Species |
Escherichia coli bacteriophage T7 |
| Depositors |
Brookhaven National Laboratory, FW Studier, Brookhaven National Laboratory |
| Applications |
produces protein RNA polymerase |
| Vector |
Construct size (kb): 7.099999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: bacteriophage T7
Gene symbol: gene 1
Genomic copy number: unique
Gene name: RNA polymerase
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: Modification: BamHI linkers
Insert end: Modification: BamHI linkers
Insert size (kb): 2.696
Cross references: DNA Seq. Acc.: J02518 Insert lengths(kb): 2.696000099182129 Gene product: RNA polymerase [gene 1] Target Gene: RNA polymerase |
| Insert Size (kb) |
2.696 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 2.8; BglII--uncut; EcoRI--7.2; PstI--7.2; HindIII--7.2. The plasmid has no T7 promoter activity. The insert includes 26 nucleotides of the natural gene 1 mRNA before the ATG initiation codon, 19 nucleotides from the natural mRNA beyond the gene 1 termination codon and has the natural ribosome-binding/translation initiation site. The insert extends from nucleotides 3145 to 5841 of T7 DNA. Gene 1 was sequenced from T7 wild-type DNA. The junctions between gene 1 and pBR322 in pAR1151 were sequenced. This produces very low levels of T7 RNA polymerase, enough to complement a T7 gene 1 amber mutant. |
| References |
Studier FW, et al. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. US Patent 4,952,496 dated Aug 28 1990
Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189: 113-130, 1986. PubMed: 3537305
Davanloo P, et al. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 81: 2035-2039, 1984. PubMed: 6371808
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
