Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 1.1; HindIII--4.4; PstI--4.4. Plasmid linearized with PstI can be used to introduce cDNA libraries constructed in pcD or pcD2 vectors into Schizosaccharomyces pombe, resulting in the formation, by homologous recombination, of concatamers maintained as episomal DNA. Shares pBR322 sequences from the PvuII site to the EcoRI site with pcD. Contains the ars sequence from Schizosaccharomyces pombe and the URA3 gene from Saccharomyces cerevisiae. Insertions at the PstI site inactivate ampicillin resistance. |
