| Vector |
Construct size (kb): 16.20000076293945 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBlueBac
Intact vector size: 14.000
Type of vector: phagemid
Vector end: NheI
Vector end: NheI
Cloning sites: NheI
Polylinker sites:
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
insert detection: lac
marker(s): ampR
promoter: polyhedrin
replicon: pMB1, f1, SV40
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCE
Gene name: protein kinase C, epsilon
Contains complete coding sequence?: Y
Tissue: brain
Type of DNA: cDNA
Insert end: NheI
Insert end: NheI
Insert size (kb): 2.2
Cross references: DNA Seq. Acc.: X65293 Insert lengths(kb): 2.200000047683716 Tissue: brain Gene product: protein kinase C, epsilon [PRKCE] Target Gene: protein kinase C, epsilon |
| Comments |
Restriction digests of the clone give the following sizes (kb): NheI--14.0, 2.3; HindIII--9.5, 4.4, 1.1, 1.0, 0.4; PstI--14.0, 1.3, 0.8, 0.2; PvuII--3.5, 3.1, 2.5, 2.4, 1.6, 1.4, 1.25, 0.4; SmaI--14.0, 2.1. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--1.92; PstI--0.32, 1.54, 1.74; PvuII--1.16. Constructed from the 5'NcoI/3'EcoRI fragment (nt 14-2185 of the sequence record) using NheI adaptors. |
| References |
Basta P, et al. Sequence and expression of human protein kinase C-epsilon. Biochim. Biophys. Acta 1132: 154-160, 1992. PubMed: 1382605
David J Burns, personal communication
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