| 產品名稱 |
[NEB#675] |
| 商品貨號 |
B236127 |
| Designations |
[NEB#675] |
| Species |
Nocardia corallina (Bergey et al.) Waksman and Henrici |
| Depositors |
New England Biolabs, Inc. |
| Applications |
produces protein modification methylase NcoI produces protein restriction endonuclease NcoI |
| Insert |
DNA: genomic Gene product: restriction endonuclease NcoI [hsdM] Target Gene: modification methylase NcoI, restriction endonuclease NcoI |
| Media |
ATCC Medium 1065 (see below) plus ampicillin (50 mcg/ml) plus chloramphenicol (20 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
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| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of one plasmid give the following sizes (kb): BamHI--6.0, 3.5; EcoRI--6.0, 3.0, 0.75; HindIII--9.8. Restriction digests of one plasmid give the following sizes (kb): BamHI--uncut; EcoRI--4.1; HindIII--4.1. This strain contains 2 plasmids. pEV190M302,325-1 expresses the NcoI methylase and pre-modifies E. coli ER1451. pEV190R612-22C-29 expresses the NcoI restriction endonuclease inducible by IPTG. The promoter, ribosome-binding site, and the initiation codon were modified to permit expression in E. coli. |
| References |
Van Cott EM. Method for cloning and producing the NcoI restriction endonuclease and methylase. US Patent 5,202,248 dated Apr 13 1993
Van Cott EM, Wilson GG. Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems. Gene 74: 55-59, 1988. PubMed: 3074019
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| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
