| 產品名稱 |
pGIKS-BioD |
| 商品貨號 |
B235360 |
| Designations |
pGIKS-BioD |
| GenBank Number |
J04423
5597)">J04423 (4947->5597)
|
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
M Follettie |
| Applications |
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). |
| Vector |
Construct size (kb): 3.56 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: KpnI
Vector end: SacI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references: Vector end (5'): KpnI Vector end (3'): SacI Excise insert: XhoI+SmaI Promoter giving the sense strand: T3 Promoter giving the antisense strand: T7 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: bioD
Gene name: dethiobiotin synthetase
Contains complete coding sequence?: N
Type of DNA: genomic
Insert 5' end: XhoI Modification: KpnI linker
Insert 3' end: SmaI Modification: SacI linker
Insert size (kb): 0.66
Cross references: DNA Seq. Acc.: J04423
Nucleotides 1-651 of the insert correspond to
nucleotides 4947-5597 of J04423. Insert lengths(kb): 0.66 Gene product: dethiobiotin synthetase [bioD] Insert end (5'): KpnI linker Insert end (3'): SacI linker |
| Insert Size (kb) |
0.660 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). The insert contains nt 4947-5597 of Genbank J04423. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.18; PvuII--0.18; RsaI--0.64. The following restriction enzymes are recommend for linearizing the plasmid for in vitro transcription: T3 = SmaI; T7 = XhoI. Restriction digests of the clone give the following sizes (kb): SmaI--3.6; SmaI/XhoI--3.0, 0.6; XhoI--3.6. |
| Classification |
Enterobacteriaceae, Escherichia |
