ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.7; PstI--2.8, 2.05, 0.55, 0.35; XhoI--5.7; BamHI--4.5, 1.1; HindIII--5.7.
The insert includes the following restriction sites (kb from the 5' end): PstI--0.21, 0.72, 2.68; XhoI--1.83; BamHI--1.86, 2.91; SacI--2.72, 2.82.
Extends from nt 410 to the EcoRI site at nt 3584, beginning at the codon for aa 114. Does not include 93 bp in the 5' coding region identified in some clones.
References
Heim R, et al. Microdiversity of human-plasma-membrane calcium-pump isoform 2 generated by alternative RNA splicing in the N-terminal coding region. Eur. J. Biochem. 205: 333-340, 1992. PubMed: 1313367
