| 產品名稱 |
pEM302 [pHUMDUT1] |
| 商品貨號 |
B234778 |
| Designations |
pEM302 [pHUMDUT1] |
| GenBank Number |
M89913
|
| Species |
Homo sapiens, human |
| Depositors |
EM McIntosh |
| Applications |
in another host, produces protein dUTP pyrophosphatase |
| Vector |
Construct size (kb): 3.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTZ19R
Intact vector size: 2.900
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC19, Escherichia coli lacZalpha, pBR322, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DUT*
Genomic copy number: multiple
Gene name: dUTP pyrophosphatase (dUTPase)
Contains complete coding sequence?: Y
Chromosome: 15; Localization: 15, cR
Type of DNA: cDNA
Insert end: Modification: EcoRI adapters
Insert end: Modification: EcoRI adapters
Insert size (kb): 0.6
Cross references: DNA Seq. Acc.: M89913 Insert lengths(kb): 0.6000000238418579 Gene product: dUTP pyrophosphatase [DUT*] Target Gene: dUTP pyrophosphatase |
| Insert Size (kb) |
0.600 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): XmnI--2.0, 1.5; SmaI--3.0, 0.5; SphI--2.95, 0.55; EcoRI--2.9, 0.6; HindIII--3.5. The insert contains the following restriction sites (nt from the 5' end): SphI--17, 86; SmaI--134. Contains the complete coding sequence, and corresponds to nt 1-545 of the database record. The antisense strand is packaged. pEM301 (ATCC 79032/3) and pEM302 (79034/5) differ only in the orientation of the insert. |
| References |
McIntosh EM, et al. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc. Natl. Acad. Sci. USA 89: 8020-8024, 1992. PubMed: 1325640
Evan M McIntosh, personal communication
|
