| 產(chǎn)品名稱(chēng) |
Vaccinia virus |
| 商品貨號(hào) |
B234389 |
| Classification |
Poxviridae, Orthopoxvirus |
| Agent |
Vaccinia virus |
| Strain |
Elstree (Lister Vaccine) |
| Biosafety Level |
2 Vaccination recommended for laboratory staff manipulating vaccinia, monkeypox, or cowpox viruses (Biosafety in Microbiological and Biomedical Laboratories, 5th edition. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2009.)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Derived from bacterially contaminated ATCC® VR-862™. Treated with multiple freeze thaws and filtrations (0.45 µm filters twice and 0.2 µm filter once) to remove bacterial contaminants. |
| Effect on Host |
In cell culture, cells appear swollen and syncytial, with eventual rounding and sloughing of cells; forms plaques. On chorio-allantoic membrane (CAM) virus forms pocks. |
| Recommended Host |
Vero cells (ATCC® CCL-81™?) |
| Growth Conditions |
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus diluted with VGM to provide a MOI of about 0.1. Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding VGM.
Incubation: Incubate infected Vero cell culture for 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance through 90% of the culture. Incubate for 3 days on CAM. |
| Name of Depositor |
JH Nakano and ATCC |
| Special Collection |
Orthopoxvirus |
| Chain of Custody |
Deposited as Vaccinia virus, Orthopoxvirus |
| Source |
ATCC® VR-1549™ is derived from bacterially contaminated ATCC® VR-862™. Treated with multiple freeze thaws and filtrations (0.45 µm filters twice and 0.2 µm filter once) to remove bacterial contaminants. ATCC® VR-862™ was originally derived from sheep. |
