ATCC trials indicate titer of frozen virus remains unchanged after three years at -80°C. Lyophilized virus may be more stable.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
The virus is mildly pathogenic for young calves. Titer of the frozen virus may decrease with prolonged storage, however, virus can be recovered by using large volumes of inoculum. ATCC trials indicate titer of frozen virus remains unchanged after three years at -80°C. Lyophilized virus may be more stable. The virus replicates poorly in MDBK cells.
Effect on Host
CPE (syncytia, cell rounding, and sloughing)
Recommended Host
BT Cells ( ATCC® CRL-1390™)
Growth Conditions
Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 70% of the monolayer.
Name of Depositor
HD Lehmkuhl
Special Collection
ATCC
Source
Clinical specimen, nasal secretions from calves in herd with signs of acute respiratory tract disease, Iowa, United States
References
Lehmkuhl HD, et al. Characterization and identification of a bovine respiratory syncytial virus isolated from young calves. Am. J. Vet. Res. 40: 124-126, 1979. PubMed: 453673
