| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--4.6, 3.0; HindIII--3.5, 1.4, 1.3, 1.2; BglI--2.7, 1.7, 1.5 (doublet). Insert contains the following restriction sites (approximate kb from the 5' end): BglI--2.66, 4.29; BglII--3.36; EcoRI--2.14; EcoRV--1.07, 1.49, 1.57, 3.19, 4.03; HindIII--0.62, 1.80, 3.22; KpnI--3.89; NdeI--0.64, 1.58, 3.52, 3.63; PvuII--2.49, 3.33. Contains entire coding sequence plus 20 nucleotides of 5' noncoding sequence. Virus was isolated from cucumber, Cucumis sativus (cultivar strain 8). |
| References |
Rochon DM, Tremaine JH. Complete nucleotide sequence of the cucumber necrosis virus genome. Virology 169: 251-259, 1989. PubMed: 2705296
D'Ann M Rochon, personal communication
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