| 產品名稱 | MiF-6 [Mutatect] |
|---|---|
| 商品貨號 | B233576 |
| Organism | Mus musculus, mouse |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 2 Cells contain SV-40 and CMV viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | fibrosarcoma |
| Gender | male |
| Strain | C57BL/10 |
| Applications | tumor model |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The Mutatect mouse tumor model is a series of cell lines (eight are available from ATCC) derived from a murine fibrosarcoma that can grow in culture as well as form subcutaneous tumors in syngeneic C57BL/6 mice. MiF-6 (ATCC CRL-2802) and M-7 (ATCC CRL-2804) are derivatives of MC-TGS17-51 (ATCC CRL-2799). M7 cells were constructed by transfection of MC-TGS17-51 cells with the pCR3.1 vector alone. The vector contains cytomegalovirus (CMV) and SV40 viral sequences and the neomycin resistance gene. M7 cells are used as a control for MiF-6. MiF-6 cells were constructed by transfection of MC-TGS17-51 cells with pRNAi-5 ligated to pCR3.1 to produce a construct expressing mouse double stranded short-interfering ribonucleic acid (siRNA) formaldehyde dehydrogenase (FDH) mRNA. The pRNAi-5 construct targeted the sequence from -18 to +4 of the mRNA, including the start codon. M7 and MiF-6 cells are 6-thioguanine sensitive, neomycin (G418) resistant, hypoxanthine phosphoribosyl transferase positive (HPRT+) and will grow in HAT selection medium. The Mutatect cell lines can be used in vitro/in vivo for the detection of deletion mutations of the hypoxanthine phosphoribosyl transferase (HPRT) gene. They are also useful for the study of inflammatory infiltration of solid tumors. |
| Clinical Data | male |
| Genes Expressed | mouse siRNA formaldehyde dehydrogenase (FDH) |
| Cellular Products | mouse siRNA formaldehyde dehydrogenase (FDH) |
| Tumorigenic | Yes |
| Effects | Yes, forms subcutaneous tumors in syngeneic C57BL/6 mice. |
| Comments | The Mutatect mouse tumor model is a series of cell lines (eight are available from ATCC) derived from a murine fibrosarcoma that can grow in culture as well as form subcutaneous tumors in syngeneic C57BL/6 mice. MiF-6 (ATCC CRL-2802) and M-7 (ATCC CRL-2804) are derivatives of MC-TGS17-51 (ATCC CRL-2799). M7 cells were constructed by transfection of MC-TGS17-51 cells with the pCR3.1 vector alone. The vector contains cytomegalovirus (CMV) and SV40 viral sequences and the neomycin resistance gene. M7 cells are used as a control for MiF-6. MiF-6 cells were constructed by transfection of MC-TGS17-51 cells with pRNAi-5 ligated to pCR3.1 to produce a construct expressing mouse double stranded short-interfering ribonucleic acid (siRNA) formaldehyde dehydrogenase (FDH) mRNA. The pRNAi-5 construct targeted the sequence from -18 to +4 of the mRNA, including the start codon. M7 and MiF-6 cells are 6-thioguanine sensitive, neomycin (G418) resistant, hypoxanthine phosphoribosyl transferase positive (HPRT+) and will grow in HAT selection medium. The Mutatect cell lines can be used in vitro/in vivo for the detection of deletion mutations of the hypoxanthine phosphoribosyl transferase (HPRT) gene. They are also useful for the study of inflammatory infiltration of solid tumors. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Protocol:
Interval: Maintain cultures at a cell concentration between 5 X 10(3) and 3 X 10(5) cells/cm2 Subcultivation Ratio: A subcultivation of 1:4 to 1:6 is recommended Medium Renewal: Two to three times weekly |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Population Doubling Time | 22 hours |
| Name of Depositor | HC Birnboim |
| Year of Origin | 2003 |
| References | Haqqani AS, et al. The role of a formaldehyde dehydrogenase-glutathione pathway in protein S-nitrosation in mammalian cells. Nitric Oxide 9: 172-181, 2003. PubMed: 14732341 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
