| Comments |
The insert contains EcoRI/HindIII fragments of the following sizes (kb): 4.574, 4.004, 3.960, 3.960, 3.028, 2.662, 2.533, 2.337, 2.184, 1.934, 1.843, 1.700, 1.655, 1.394, 1.338, 1.019, 0.759, 0.555, 0.552, 0.440. In an EcoRI/HindIII digest of the cosmid, the vector fragments are (kb): 7.966, 3.960. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70375, 70674, 70682, 70998, 71056. NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us. |
