| Medium |
LYI Entamoeba medium Casein Digest (BBL 97023)*, 20.0 g Yeast Extract (BBL 11928)*, 10.0 g NaCl, 2.0 g L-Cysteine . HCl (Sigma C7880), 1.0 g Ascorbic Acid (J.T. Baker B582-5), 0.2 g Ferric Ammonium Citrate (Mallinckrodt 0658), 22.8 mg Distilled water, 770.0 ml Adjust pH to 6.8 with NaOH. Autoclave at 121C for 15 minutes. Cool to room temperature and aseptically add the following solutions: Solution A: Dextrose, 10.0 g Distilled water, 50.0 ml Filter-sterilize. Solution B: K2HPO4, 1.0 g KH2PO4, 0.6 g Distilled water, 50.0 ml Autoclave at 121C for 15 minutes. Solution C: Bovine serum (heat-inactivated), 100.0 ml Solution D: Special 107 Vitamin Mix (see below), 30.0 ml Special 107 Vitamin Mix: Aseptically combine the following: Solution 1 (see below), 0.4 ml Solution 2 (see below), 1.2 ml Solution 3 (see below), 0.4 ml Sterile distilled water, 18.0 ml Solution 4 Vitamins (see below), 100.0 ml Total volume of complete Special 107 Vitamin Mix is 120 ml. Solution 1 (filter-sterilized): D,L-6,8-Thioctic acid, oxidized (Sigma T1395), 100.0 mg Absolute Ethanol, 100.0 ml Solution 2 (filter-sterilized): Vitamin B12 (Sigma V2876), 40.0 mg Distilled water, 100.0 ml Solution 3 (filter-sterilized): Tween 80 (Sigma P1754), 50.0 g Absolute Ethanol, 100.0 ml Solution 4 Vitamins: alpha Tocopherol phosphate, disodium salt, 0.025 mg d-Biotin, 0.025 mg Calciferol (Vitamin D2), 0.250 mg Calcium D-(+)-pantothenate, 0.025 mg Choline chloride, 1.250 mg Folic acid, 0.025 mg i-Inositol, 0.125 mg Menadione (Vitamin K3), 0.025 mg Niacin, 0.0625 mg Niacinamide, 0.0625 mg p-Aminobenzoic acid, 0.125 mg Pyridoxal . HCl, 0.0625 mg Pyridoxine . HCl, 0.0625 mg Riboflavin, 0.025 mg Thiamine . HCl, 0.025 mg Vitamin A (crystallized alcohol), 0.250 mg Vitamin B12, 10.000 mg Distilled water, 1.0 L *Some lots of Casein Digest, Yeast Extract, or serum may inhibit growth.7071 LYI Entamoeba medium Casein Digest (BBL 97023)*, 20.0 g Yeast Extract (BBL 11928)*, 10.0 g NaCl, 2.0 g L-Cysteine . HCl (Sigma C7880), 1.0 g Ascorbic Acid (J.T. Baker B582-5), 0.2 g Ferric Ammonium Citrate (Mallinckrodt 0658), 22.8 mg Distilled water, 770.0 ml Adjust pH to 6.8 with NaOH. Autoclave at 121C for 15 minutes. Cool to room temperature and aseptically add the following solutions: Solution A: Dextrose, 10.0 g Distilled water, 50.0 ml Filter-sterilize. Solution B: K2HPO4, 1.0 g KH2PO4, 0.6 g Distilled water, 50.0 ml Autoclave at 121C for 15 minutes. Solution C: Bovine serum (heat-inactivated), 100.0 ml Solution D: Special 107 Vitamin Mix (see below), 30.0 ml Special 107 Vitamin Mix: Aseptically combine the following: Solution 1 (see below), 0.4 ml Solution 2 (see below), 1.2 ml Solution 3 (see below), 0.4 ml Sterile distilled water, 18.0 ml Solution 4 Vitamins (see below), 100.0 ml Total volume of complete Special 107 Vitamin Mix is 120 ml. Solution 1 (filter-sterilized): D,L-6,8-Thioctic acid, oxidized (Sigma T1395), 100.0 mg Absolute Ethanol, 100.0 ml Solution 2 (filter-sterilized): Vitamin B12 (Sigma V2876), 40.0 mg Distilled water, 100.0 ml Solution 3 (filter-sterilized): Tween 80 (Sigma P1754), 50.0 g Absolute Ethanol, 100.0 ml Solution 4 Vitamins: alpha Tocopherol phosphate, disodium salt, 0.025 mg d-Biotin, 0.025 mg Calciferol (Vitamin D2), 0.250 mg Calcium D-(+)-pantothenate, 0.025 mg Choline chloride, 1.250 mg Folic acid, 0.025 mg i-Inositol, 0.125 mg Menadione (Vitamin K3), 0.025 mg Niacin, 0.0625 mg Niacinamide, 0.0625 mg p-Aminobenzoic acid, 0.125 mg Pyridoxal . HCl, 0.0625 mg Pyridoxine . HCl, 0.0625 mg Riboflavin, 0.025 mg Thiamine . HCl, 0.025 mg Vitamin A (crystallized alcohol), 0.250 mg Vitamin B12, 10.000 mg Distilled water, 1.0 L *Some lots of Casein Digest, Yeast Extract, or serum may inhibit growth.
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| References |
Diamond LS. A new Monocercomonas from the water snake, Natris sipedon. J. Protozool. 6: 8, 1959.
Gunderson J, et al. Phylogeny of trichomonads inferred from small-subunit rRNA sequences. J. Eukaryot. Microbiol. 42: 411-415, 1995. PubMed: 7620466
Delgado-Viscogliosi P, et al. Electrophoretic mobility of tubulin subunits as a criterion for testing relationships between trichomonad taxa. Arch. Protistenkd. 146: 191-200, 1995.
Viscogliosi E, et al. Phylogenetic implication of iron-containing superoxide dismutase genes from trichomonad species. Mol. Biochem. Parasitol. 80: 209-214, 1996. PubMed: 8892298
Keeling PJ, et al. Evolutionary relationship between translation initiation factor eIF-2gamma and selenocysteine-specific elongation factor SELB: change of function in translation factors. J. Mol. Evol. 47: 649-655, 1998. PubMed: 9847405
Viscogliosi E, Mueller M. Phylogenetic relationships of the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, from parabasalid flagellates. J. Mol. Evol. 47: 190-199, 1998. PubMed: 9694668
Gerbod D, et al. Phylogenetic relationships of class II fumarase genes from trichomonad species. Mol. Biol. Evol. 18: 1574-1584, 2001. PubMed: 11470849
Brugerolle G, et al. Immunolocalization of two hydrogenosomal enzymes of Trichomonas vaginalis. Parasitol. Res. 86: 30-35, 2000. PubMed: 10669133
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| Cross References |
Nucleotide (GenBank) :
U17511
SSU rRNA
Nucleotide (GenBank) :
Z70667
Monocercomonas sp. sod gene.
Nucleotide (GenBank) :
U17507
Monocercomonas sp. ATCC 50210 16S-like rRNA gene.
Nucleotide (GenBank) :
U66902
Monocercomonas ATCC50210 alpha-tubulin (atub1) gene, partial cds.
Nucleotide (GenBank) :
U66903
Monocercomonas ATCC50210 alpha-tubulin (atub2) gene, partial cds.
Nucleotide (GenBank) :
AF022420
Monocercomonas sp. glyceraldehyde-3-phosphate dehydrogenase (gap) gene, partial cds.
Nucleotide (GenBank) :
AF005077
Monocercomonas ATCC50210 translation initiation factor 2 gamma subunit (eIF-2 gamma) gene, partial cds.
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