ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 2.1, 0.68; SalI--5.8; HindIII--5.8; PstI--4.6, 1.35; BglII--5.8.
The insert includes 2498 bp followed by 92 adenylates. The insert is located downstream from a SV40 enhancer, early promoter and 16S and 19S mRNA splice sites, and upstream from SV40 poly(A) signal.
The insert contains a BamHI site. The 2.0 kb BamHI fragment contains all the coding sequences of the mRNA, plus some pcD sequence at the 5' end.
A 2.0 kb BamHI fragment was used to detect the RFLP.
References
Clarke MF, et al. Transformation of NIH 3T3 cells by a human c-sis cDNA clone. Nature 308: 464-467, 1984. PubMed: 6323994
Josephs SF, et al. Transforming potential of human c-sis nucleotide sequences encoding platelet-derived growth factor. Science 225: 636-639, 1984. PubMed: 6740330
Ratner L, et al. Nucleotide sequence of transforming human c-sis cDNA clones with homology to platelet-derived growth factor. Nucleic Acids Res. 13: 5007-5018, 1985. PubMed: 2991848
