Total size of construct (kb): 29.732
Marker: tetR
Vector: pACYC184
Vector type: cloning vector
Insert site: EcoRI
Vector size (kb): 4.245
Suggested primer for sequencing from the 5’ side of the EcoRI site:
Primer yE-1 (forward):
5’-GTCGTGGTATTCACTCCAGAGCGAT-3’
Primer yE-2 (reverse):
5’-GCATTTCAGTCAGTTGCTCAATGTACCTATA-3’
Insert
Insert size (kb): 25.487
Insert ends: EcoRI
DNA: partial genomic DNA
Source: Molluscum contagiosum virus (MCV)
Genbank accession: U60315, partial
Nucleotides 1-25487 of the insert correspond to nucleotides 83880-109367 of Genbank U60315
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed as 200 ng purified plasmid DNA
Comments
This is a partial clone of the Molluscum contagiosum virus in a low copy number plasmid. The insert is 25.487 kb and contains an internal EcoRI site. Sequence of the insert corresponds to nucleotides 83880-109367 of Genbank sequence U60315 for the complete genome of the virus. The internal EcoRI site is at nt 107692.
References
Bugert JJ, Darai G. Stability of molluscum contagiosum virus DNA among 184 patient isolates: evidence for variability of sequences in the terminal inverted repeats. J. Med. Virol. 33(3): 211-217, 1991. PubMed: 1880497
Melquiot NV, Bugert JJ. Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens. Methods Mol. Biol. 269: 371-384, 2004. PubMed: 15114026
Senkevich TG, et al. Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes. Science 273(5276): 813-816, 1996. PubMed: 8670425
