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18-26-4

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編號(hào):B231461

品牌:Mingzhoubio

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產(chǎn)品名稱(chēng)	18-26-4
商品貨號(hào)	B231461
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue	B lymphocyte; hybridoma
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Dengue
Applications	The antibody binds specifically to an idiotypic determinant on 3H5 anti-Dengue 2 antibody. Dengue 2 virus inhibits the binding of 18-26-4 antibody to 3H5. The antibody reacts with sera from mice immunized to Dengue 2 virus and also with human antibody in acute and convalescent human dengue patient samples.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Animals were immunized with 3H5-1 (ATCC HB-46), a monoclonal antibody to Dengue 2 virus, conjugated to KLH (keyhole limpet hemocyanin). Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody binds specifically to an idiotypic determinant on 3H5 anti-Dengue 2 antibody. Dengue 2 virus inhibits the binding of 18-26-4 antibody to 3H5. The antibody reacts with sera from mice immunized to Dengue 2 virus and also with human antibody in acute and convalescent human dengue patient samples.
Clinical Data	Dengue 2 virus inhibits the binding of 18-26-4 antibody to 3H5. The antibody reacts with sera from mice immunized to Dengue 2 virus and also with human antibody in acute and convalescent human dengue patient samples.
Genes Expressed	immunoglobulin; monoclonal antibody; against an idiotypic determinant on 3H5-1 (monoclonal antibody against Dengue virus-2)
Cellular Products	immunoglobulin; monoclonal antibody; against an idiotypic determinant on 3H5-1 (monoclonal antibody against Dengue virus-2)
Comments	Animals were immunized with 3H5-1 (ATCC HB-46), a monoclonal antibody to Dengue 2 virus, conjugated to KLH (keyhole limpet hemocyanin). Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody binds specifically to an idiotypic determinant on 3H5 anti-Dengue 2 antibody. Dengue 2 virus inhibits the binding of 18-26-4 antibody to 3H5. The antibody reacts with sera from mice immunized to Dengue 2 virus and also with human antibody in acute and convalescent human dengue patient samples.
Complete Growth Medium	Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10⁵ viable cells/mL. Maintain cultures at a cell concentration between 2 x 10⁵ and 1 x 10⁶ cells/mL. Do not allow the cell concentration to exceed 1 x 10⁶ cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgM
Name of Depositor	D.G. Sieckmann
References	Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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3	ATCC代理	查看詳情
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