Restriction digests of the clone give the following sizes (kb): BamHI--2.75, 2.7; KpnI--4.9, 0.5; PstI--3.15, 1.4, 0.87; SacI--5.4.
The insert contains the following restriction sites (approximate kb from a BamHI site): EcoRI--2.17; KpnI--0.23, 0.69; PstI--0.42, 1.32; SacI--0.94.
Features of the virus occupy the following positions: LIR--(2-233); SIR--(1376-1579); ORF 33.1--(2750-1867); ORF 15.8--(1987-1580); ORF 26.8--(658-1335); ORF 14.5--(234-1375).
Constructed by linearizing the viral ds DNA at the unique BamHI site (nt 351) within the ORF encoding a 14.5 kDa protein. |
