| Applications |
in another host, produces protein mitogen-activated protein kinase kinase protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), MAP kinase kinase |
| Vector |
Construct size (kb): 6.454999923706055 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDNA I/Amp
Intact vector size: 5.000
Type of vector: phagemid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: HindIII BamHI BstXI EcoRI EcoRV BstXI NotI XhoI SphI NsiI
XbaI
Polylinker sites: HindIII BamHI BstXI EcoRI EcoRV BstXI NotI XhoI SphI NsiI
XbaI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: CMV
marker(s): ampR
promoter: CMV, T7, SP6
replicon: polyoma, ColE1, M13
terminator: SV40
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MKK*
Gene name: mitogen-activated protein kinase kinase
Contains complete coding sequence?: U
Tissue: T-lymphocyte
Type of DNA: cDNA
Insert 5' end: BamHI
Insert 3' end: EcoRI
Insert size (kb): 1.455
Cross references: DNA Seq. Acc.: L05624 Insert lengths(kb): 1.455000042915344 Tissue: T-lymphocyte Gene product: mitogen-activated protein kinase kinase( protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), MAP kinase kinase) [MKK*] Target Gene: mitogen-activated protein kinase kinase |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRV--5.0, 1.45; BglII--6.5; EcoRI--5.5, 1.0; PvuII--2.8, 1.95, 1.7; HaeII--3.6, 2.2, 0.7. The insert contains the following restriction sites (bp from the 5' end): AflIII--719; AlwI--486; BanI--860; BglII--1120; BspMII--353; BsrI--1010; BstUI--15; EcoRI--512; HaeII--176; HgiAI--463; HpaI--91; HphI--1314; PvuII--61; RsaI--405; SspI--1374; TaqI--680. A probe from nt 1-516 of the insert detects a 2.6 kb mRNA in human thymus, brain, kidney, muscle, liver, intestine, heart, lung, spleen and EGF-stimulated A431 cells. |
| References |
Seger R, et al. Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases. J. Biol. Chem. 267: 25628-25631, 1992. PubMed: 1281467
Edwin G Krebs, personal communication
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