| Vector |
Construct size (kb): 3.799999952316284 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pIBI24
Intact vector size: 2.878
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI
XhoI ApaI mLuI SphI HindIII
Polylinker sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI
XhoI ApaI mLuI SphI HindIII
Construction: pEMBL
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GZMB
Other gene symbols: CTLA1; CSPB; CCPI; CGL-1
Genomic copy number: unique
Gene name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated
serine esterase 1)
Contains complete coding sequence?: Y
Chromosome: 14; Localization: 14 q11.2
Tissue: T lymphocyte, activated
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.9
Cross references: DNA Seq. Acc.: J04071 Insert lengths(kb): 0.8999999761581421 Tissue: T lymphocyte, activated Gene product: cytotoxic serine protease B [CCPI] |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglI--2.05, 1.35, 0.66; EcoRI--3.15, 0.92; HindIII--3.2, 0.82; PstI--3.65, 0.39; PvuII--2.7, 0.76, 0.36, 0.20; SalI--3.95. Contains the complete coding sequence (nt 17-760). The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.2, 0.35; ApaI--0.22; PstI--0.35; BglI--0.4; TaqI--0.56; HindIII--0.72. Detects genomic restriction fragments of the following sizes (approximate kb): human, BamHI--12; human, BglII--14; human, BclI--10; human, EcoRV--18; human, TaqI--3.5, 2.5; human, PstI--4.0, 2.2; mouse, PstI--4.0. |
| References |
Trapani JA, et al. Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes. Proc. Natl. Acad. Sci. USA 85: 6924-6928, 1988. PubMed: 3261871
Klein JL, et al. Genomic organization and chromosomal assignment for a serine protease gene (CSPB) expressed by human cytotoxic lymphocytes. Genomics 5: 110-117, 1989. PubMed: 2788607
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