DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: PstI Vector end: PstI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: HLA-DRB* Gene name: major histocompatibility complex, class II, DR beta Contains complete coding sequence?: Y Chromosome: 6; Localization: 6 p21.3 Tissue: B-lymphocyte IBW 9 cell line Type of DNA: cDNA Insert end: Modification: GC tails Insert end: Modification: GC tails Insert size (kb): 1.2 Cross references:
Insert lengths(kb): 1.200000047683716
Tissue: B lymphocyte, IBW 9 cell line
Gene product: major histocompatibility complex, class II, DR beta 1(HLA class II histocompatibility antigen, DR-1 beta chain) [HLA-DRB*]
Insert Size (kb)
1.200
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.2; HindIII--5.2, 1.2; PstI--5.8, 0.6.
The cell line IBW 9 is heterozygous at DR4, w6.
References
Mach BF, et al. DNA sequences coding for the DR-beta-chain locus of the human lymphocyte antigen complex and polypeptides, diagnostic typing processes and products related thereto. US Patent 5,169,941 dated Dec 8 1992
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
