Restriction digests of the clone give the following sizes (kb): PstI--4.25; BamHI--4.4; EcoRI--3.2, 1.35; SmaI--4.45; SalI--4.4. This clone maps about 6.5 kb upstream to MYF5 and is in the same orientation. This clone includes the complete coding sequence (nucleotides 55 - 780) plus 5' and 3' untranslated regions. This clone detects a genomic EcoRI fragment of about 3.5 kb and mRNA of 2.3 and 1.7 kb. |
