| 產品名稱 |
GMCSF [pCSF-1] |
| 商品貨號 |
B230181 |
| Designations |
GMCSF [pCSF-1] |
| Species |
Homo sapiens, human |
| Depositors |
Genetics Institute, Inc., GG Wong, Genetics Institute, Inc. |
| Applications |
in another host, produces protein colony stimulating factor 2 (granulocyte-macrophage) |
| Vector |
Construct size (kb): 9.75 Name of vector: p91023(B)
Intact vector size: 8.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: SV40
marker(s): tetR
promoter: Ad2 major late
replicon: pMB1, SV40
terminator: SV40 |
| Insert |
DNA: cDNA Genome: human
Gene symbol: CSF2
Genomic copy number: unique
Gene name: colony stimulating factor 2 (granulocyte-macrophage)
Contains complete coding sequence?: U
Chromosome: 5; Localization: 5 q23-q31
Tissue: T-lymphocyte Mo cell line (ATCC CRL 8066)
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.8 Insert lengths(kb): 0.80 Tissue: T-lymphocyte Mo cell line (ATCC CRL 8066) Gene product: colony stimulating factor 2 (granulocyte-macrophage) [CSF2] Target Gene: colony stimulating factor 2 (granulocyte-macrophage) |
| Insert Size (kb) |
0.800 |
| Media |
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: frozen |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--8.0, 0.81; HindIII--4.8, 1.6, 1.3, 0.6. This clone may be most reliably recovered by streaking on tetracycline-containing plates, picking single colonies, and doing a miniprep. Contains 8 bp 5' untranslated; ORF of 432 nucleotides encoding 144 amino acids (complete coding sequence), and 3' untranslated sequences. The vector allows expression in COS cells. The insert contains PstI, BglI, NcoI and AhaIII sites. |
| References |
Clark SC, et al. Method for identification and isolation of DNA encoding a desired protein. US Patent 4,675,285 dated Jun 23 1987
Rajagopalan LE, Malter JS. Turnover and translation of in vitro synthesized messenger RNAs in transfected, normal cells. J. Biol. Chem. 271: 19871-19876, 1996. PubMed: 8702698
Wong GG, et al. Human GM-CSF: Molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science 228: 810-815, 1985. PubMed: 3923623
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
