| 產(chǎn)品名稱(chēng) |
pBS-hCD40L-6A9 |
| 商品貨號(hào) |
B229904 |
| Designations |
pBS-hCD40L-6A9 |
| GenBank Number |
L07414
|
| Species |
Homo sapiens, human |
| Depositors |
J Gauchat |
| Vector |
Construct size (kb): 4.059999942779541 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7, T3
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD40LG
Other gene symbols: CD40L
Gene name: CD40 antigen ligand
Contains complete coding sequence?: Y
Chromosome: X; Localization: X q26
Tissue: CD4-positive T-cells
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: L07414 Insert lengths(kb): 1.100000023841858 Tissue: CD4-positive T-cells Gene product: CD40 antigen ligand [CD40L] |
| Insert Size (kb) |
1.100 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 0.2; HindIII--3.3, 0.7; NcoI--4.0; PvuII--2.6, 1.0, 0.6; EcoRI--3.0, 1.2. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.93; HindIII--0.58; NcoI--0.78; PvuII--0.52. Contains the complete coding sequence (nt 40-825 of the sequence record). |
| References |
Gauchat JF, et al. Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production. FEBS Lett. 315: 259-266, 1993. PubMed: 7678552
Jean-Francois Gauchat, personal communication
|
