| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PPP3CB
Other gene symbols: CALNA2
Genomic copy number: unique
Gene name: protein phosphatase 3 (formerly 2B), catalytic subunit,
beta isoform (calcineurin A beta)
Contains complete coding sequence?: U
Chromosome: 10; Localization: 10; 10, 448.339999 cR
Tissue: brainstem
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.215
Cross references: DNA Seq. Acc.: M29551 Insert lengths(kb): 1.2150000333786 Tissue: brainstem Gene product: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta)(protein phosphatase 3, catalytic subunit, beta isoform) [CALNA2] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--24.2, 19.5, 1.2; ClaI--12.5, 6 (doublet), 4.2, 4.0, 2.7, 2.6, 2.0, 1.85, 1.1 (doublet), 0.95, 0.6, 0.35; HindIII-->23.0, 9.4, 6.2, 3.8, 0.56; SmaI--19.5, 10.5, 8.3, 5.8; SstI--20.0, 18.5, 4.45, 1.35. The insert contains the following restriction sites (approximate kb from the 5' end): ClaI--0.97; HindIII--0.67, 0.68; NcoI--0.42; SacI--0.98; SmaI--0.10, 0.14, 0.18. Corresponds to nt 1-1215 of the GenBank record, including the 5' portion of the coding region (nt 117-1691). This is the sequence for the more prevalent type II isoform, and lacks 54 bp in this region relative to the type I. |
