| Insert |
DNA: cDNA Insert lengths(kb): 0.699999988079071 Tissue: T4+,T8- T-cell clone Gene product: antigen CD3D, delta polypeptide (TiT3 complex) [T3D] Alleles: B2, B2, B1, B1, , , , , , , , A1, A2, , , , , , , , A1, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--3.9; SalI--3.9; PstI--2.5, 1.3; BamHI--2.9, 0.6, 0.5. TaqI and MspI RFLP alleles are in strong linkage disequilibrium. Codominant segregation was observed in 4 unrelated families (49 individuals). The insert contains ATG at 97, TGA at 610, and 89 bp of 3' untranslated sequence including AATAA followed by poly(A) 17 bp downstream. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. Plasmid pPGBC9 was cut with BamHI and the fragments of 0.5 kb and 0.6 kb were isolated, pooled and used as the probe for the MspI and TaqI RFLPs. Enzyme(s) not detecting polymorphism: RsaI, BstEII, EcoRV, BamHI, HindIII, BglII, PvuII, BglI and PstI in at least 6 unrelated individuals. |
| References |
Malhotra U, Concannon P. Human T-cell receptor CD3-delta (CD3D) / MspI DNA polymorphism. Nucleic Acids Res. 17: 2373, 1989. PubMed: 2468134
van den Elsen P, et al. Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex. Nature 312: 413-418, 1984. PubMed: 6095101
Charmley P, et al. A primary linkage map of the human chromosome 11q22-23 region. Genomics 6: 316-323, 1990. PubMed: 2307473
Barbara Weiffenbach, personal communication
|
