| 產(chǎn)品名稱(chēng) |
pCMV5FIXS61A [pCMV5BFIXs61A] |
| 商品貨號(hào) |
B228720 |
| Designations |
pCMV5FIXS61A [pCMV5BFIXs61A] |
| GenBank Number |
J00136
|
| Species |
Homo sapiens, human |
| Depositors |
W Cheung |
| Applications |
in another host, produces protein coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Vector |
Construct size (kb): 7.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCMV5
Intact vector size: 4.700
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI BamHI HindIII
Polylinker sites:
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
promoter: CMV, ->
terminator: hGH, ->
replicon: SV40
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: F9
Genomic copy number: unique
Gene name: coagulation factor IX (plasma thromboplastic component,
Christmas disease, hemophilia B)
Contains complete coding sequence?: U
Chromosome: X; Localization: X q26.3-q27.1
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 2.8
Cross references: DNA Seq. Acc.: J00136 Insert lengths(kb): 2.799999952316284 Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) [F9] Alleles: A1, A2, D2, C1, D1, C2 Target Gene: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Insert Size (kb) |
2.800 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 3.1; EcoRI--4.9, 2.7; NotI--7.8; SstI--7.2, 0.54; SphI--3.7, 2.75, 0.36 (doublet), <0.3. Digestion with EcoRI produces fragments of 5.0 and 2.5 kb. Digestion with SphI produces fragments of 3.9, 2.7, 0.4, 0.4 and 0.08 kb. Bacteria may grow slowly due to the high plasmid copy number. Encodes the following mutant form: S61A. |
| References |
Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778
Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11. J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370
Cheung WF, et al. The role of the epidermal growth factor-1 and hydrophobic stack domains of human factor IX in binding to endothelial cells. J. Biol. Chem. 266: 8797-8800, 1991. PubMed: 1851159
Wing-Fai Cheung, personal communication
|
