| Applications |
Enzyme(s) not detecting polymorphism: EcoRI, HindIII, PstI, PvuII, SmaI, SstI, MspI, TaqI. Tested against 8 unrelated individuals. |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--3.9, 1.5; BamHI--4.3, 1.2; AvaI--5.6; HindIII--5.8; EcoRI--4.6, 0.63, 0.42. Includes unspliced intervening sequences and 3' non-coding sequences. Encodes from about aa 103 through 270 bp 3' untranslated. This is a subclone of pHUK-1. BamHI RFLP has demonstrated codominant segregation in an informative family of 7. Enzyme(s) not detecting polymorphism: EcoRI, HindIII, PstI, PvuII, SmaI, SstI, MspI, TaqI. Tested against 8 unrelated individuals. |
| References |
Verde P, et al. Identification and primary sequence of an unspliced human urokinase poly(A)+ RNA. Proc. Natl. Acad. Sci. USA 81: 4727-4731, 1984. PubMed: 6589620
Sebastio G, et al. BamHI RFLP linked to the human urokinase gene. Nucleic Acids Res. 13: 5404, 1985. PubMed: 2991863
Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050
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