Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Because of the temperature sensitive repressor, heat shock for CaCl2 transformations should be at 32C for 10 min, followed by 1 min on ice. Add LB and grow at room temperature for 1-2 hours.
Selection for ampR is on LB + amp (100 ug/ml) plates incubated at 30-32C.
An isogenic host with N gene function is TAP56, ATCC 47074.
Medium
ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C
Name of Depositor
TA Patterson
References
Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761
Patterson TA, et al. Improved bacterial hosts for regulated expression of genes from lambda PL plasmid vectors. Gene 132: 83-87, 1993. PubMed: 8406046
