| 產(chǎn)品名稱 | CHOP-KO-DR |
|---|---|
| 商品貨號 | B228262 |
| Organism | Mus musculus, mouse |
| Tissue | embryo fibroblast/embryo |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | fibroblast-like |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain SV40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | embryo, 13.5 days gestation |
| Applications | DR-Wildtype cells, CRL-2977, are available for use as a control. These cells lack the transcription factor CHOP, a key mediator of cell death in the unfolded protein response (UPR). CHOP is a major regulator of the endoplasmic reticulum UPR and as such, these CHOP-KO cells are useful for studying the mechanisms of UPR and integrated stress response. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The mouse embryonic fibroblast (MEF) cell line, CHOP-KO-DR (CRL-2979), was established from a 13.5 day-old CHOP-/- mouse embryo by SV-40 immortalization. |
| Genes Expressed | C/EBP homologous protein (CHOP)/growth arrest and DNA damage-inducible gene 153 (GADD153), not expressed |
| Cellular Products | C/EBP homologous protein (CHOP)/growth arrest and DNA damage-inducible gene 153 (GADD153), not expressed |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:10 to 1:30 twice weekly is recommended.
Medium renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: fetal bovine serum, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor | D Ron and H Harding |
| Year of Origin | 1998 |
| References | Zinszner H, et al. CHOP is implicated in programmed cell death in response to impaired function of the endoplasmic reticulum. Genes Dev. 12(7): 982-995, 1998. PubMed: 9531536 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
