Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8. The vector contains a 0.7 kb bovine cDNA that can be excised using the 5' and 3' cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression. One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences. The insert is expressed as a Met-His fusion protein. Sequences cloned into the NsiI/NdeI site are expressed as a Met-His fusion protein, while sequences cloned into the SwaI site can be expressed as an unfused protein (must provide ATG in cloned insert). |
