| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: BCR
Other gene symbols: BCR1
Genomic copy number: unique
Gene name: breakpoint cluster region
Contains complete coding sequence?: N
Chromosome: 22; Localization: 22 q11
Tissue: chronic myelogenous leukemia K-562 cell line
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 39.199
Cross references: DNA Seq. Acc.: U07000
Nucleotides 1-39199 of the insert correspond to
nucleotides 85435-124633 of U07000. Insert lengths(kb): 39.19900131225586 Tissue: chronic myelogenous leukemia K-562 cell line Gene product: breakpoint cluster region [BCR] Alleles: A1, A1, A2, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 12.5, 10.5, 7.0, 1.45, 0.52; BglII--13.5, 9.8, 7.0, 6.2, 4.8, 2.7, 2.6, 1.75, 0.92, 0.54; EcoRI--20.0, 17.0, 6.6, 2.75, 1.2, 1.1, 0.6; SstI--7.5, 6.4, 4.4, 3.8, 3.5 (doublet), 3.35, 2.15, 2.05, 1.7, 1.45, 1.3, 0.62, 0.4; XbaI--18.0 (doublet), 10.5, 2.35; SalI--33.0, 16.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert begins in intron 3' and continues into exon 15. This region includes an open reading frame (96845-97681) that appears to be expressed. |
| References |
Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859
Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185
Bruce A Roe, personal communication
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