| 產(chǎn)品名稱 |
plambdaP2 [pP2] |
| 商品貨號(hào) |
B226913 |
| Designations |
plambdaP2 [pP2] |
| GenBank Number |
J00290
|
| Species |
Homo sapiens, human |
| Depositors |
SN Cohen |
| Applications |
Enzyme(s) not detecting polymorphism: BglII, PstI, BamHI, AvaII, BglI, MspI. Tested against 20 unrelated individuals. |
| Vector |
Construct size (kb): 6.300000190734863 |
| Insert |
DNA: genomic Insert lengths(kb): 1.899999976158142 Tissue: liver Gene product: proopiomelanocortin (adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin)(adrenocorticotropic hormone (ACTH)) [POMC] Alleles: A1, A2, B1, B2 |
| Insert Size (kb) |
1.900 |
| Media |
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--6.3; HindIII--6.3; PstI--4.4, 1.9. The insert includes 5' flanking sequences and exon 1. SstI polymorphism is about 10 kb 5' to exon 1 of POMC. Shows Mendelian segregation with codominance. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. Enzyme(s) not detecting polymorphism: BglII, PstI, BamHI, AvaII, BglI, MspI. Tested against 20 unrelated individuals. |
| References |
Feder J, et al. A DNA polymorphism in close physical linkage with the proopimelanocortin gene. Am. J. Hum. Genet. 35: 1090-1096, 1983. PubMed: 6316780
Feder J, et al. DNA restriction fragment analysis of the proopiomelanocortin gene in schizophrenia and bipolar disorders. Am. J. Hum. Genet. 37: 286-294, 1985. PubMed: 2984925
Cochet M, et al. Characterization of the structural gene and putative 5'-regulatory sequences for human proopiomelanocortin. Nature 297: 335-339, 1982. PubMed: 6927850
Barbara Weiffenbach, personal communication
|
