Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.35; PstI--4.4, 1.35; PvuII--2.7, 2.1, 1.0; BglII--5.8; HindIII--5.8; EcoRI/PvuI--3.0, 1.4, 1.1, 0.2; EcoRI/PvuII--2.6, 1.4, 1.0, 0.55, 0.2. Restriction digests of the clone give the following sizes (kb): NotI/SalI--3.0, 2.7. The insert spans two adjacent genomic NotI fragments of 325 kb and 1400 kb. The 5' end is in the 325 kb NotI fragment. Corresponds to the 3' portion of the reported sequence. Overlaps ph12 (ATCC 65958, 65959). One of the EcoRI sites on the insert ends was lost during cloning. |
