| 產品名稱 |
Human adenovirus 19 |
| 商品貨號 |
B226287 |
| Classification |
Adenoviridae, Mastadenovirus, Human adenovirus D |
| Deposited As |
Adenovirus type 19 |
| Agent |
Human adenovirus 19 |
| Strain |
AV-587 [3911] |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1.0 mL |
| Storage Conditions |
-70°C or colder |
| Comments |
Replicates very slowly. Agglutinates rat RBC. Identical to adenovirus type 10 in HAI but different in neutralization tests
REFERENCE STRAIN: Pereira, H.G. et al., Virology 20: 613, 1963. |
| Effect on Host |
CPE, refractile rounding and sloughing |
| Recommended Host |
KB cells (ATCC® CCL-17™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01-0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 6-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor |
SD Bell |
| Source |
Conjunctival scrapings from child with trachoma, Saudi Arabia, 1955 |
| Year of Origin |
1955 |
| References |
Bell SD. . Am. J. Trop. Med. Hyg. 9: 523, 1960.
Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904
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