Restriction digests of the clone give the following sizes (kb): EcoRI--4.7, 4.4; PstI--7.3, 2.1; BamHI--9.4; AvaI--5.2, 4.4; PvuII--6.7, 2.7. Insert contains these sites in kb from 5' EcoRI site: PvuII--0.34; XbaI--0.4; SmaI/AvaI--1.1; PstI--1.4; HincII--1.6; HindIII--2.6; HindIII/HincII--3.3; EcoRI--4.0. The insert includes exon 5 in a 1.0 kb HincII/HindIII fragment. |
