Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.0; BamHI--4.7; HindIII--4.7; SalI--4.7; XbaI--4.7. The 900 bp probe can be excised by an EcoRI/EcoRV double digest. When transcription is controlled by an RSV long terminal repeat, the cDNA clone induced foci of transformation in NIH3T3 cells. Contains a complete coding sequence and an AT-rich 3' non-coding sequence of about 1200 bp. The 3' region includes an inverted Alu repeat, a non-canonical polyadenylation signal, and the ATTT(A) sequence of short-lived RNAs. A probe from the 5' 900 bp of this insert has been used to detect mRNA in mouse and human teratocarcinoma cells. |
