| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--4.2, 4.2, 3.0, 3.0, 3.0, 2.35, 2.05, 1.95, 1.9, 1.7, 1.5, 1.35, 1.25, 1.1, 0.92, 0.9, 0.85, 0.85, 0.8, 0.76, 0.72, 0.48, 0.42, 0.35; PvuII--4.6, 4.4, 4.1, 3.7, 3.7, 3.2, 2.2, 2.0, 1.9, 1.65, 1.25, 1.25, 1.0, 1.0, 0.75, 0.65, 0.6, 0.46, 0.4, 0.4; EcoRI--12.0, 8.0, 7.0, 4.5, 3.3, 1.65, 0.84; EcoRI/BamHI--8.4, 8.4, 4.5, 4.3, 3.65, 2.25, 1.85, 1.78, 1.7, 1.15, 0.8; EcoRI/PstI--4.2, 3.3, 3.0, 3.0, 2.35, 2.2, 1.75, 1.55, 1.35, 1.2, 1.15, 1.1, 1.0, 0.95, 0.95, 0.84, 0.75, 0.68 +smaller. The 4428 nt sequenced region includes the following restriction sites (nt from the 5' end): PstI--531, 642, 1011 (all intron 1), 1850 (intron 2); 3218 (intron 3); EcoRI--4211 (exon 4). IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The sequence record begins at a Sp1 binding site consensus approximately 260 bp 5' to the initiator ATG. Contains the complete gene (4 exons). Introns 2 and 3 contain Alu repeats. |
