Restriction digests of the clone give the following sizes (kb): SacI--5.0; PvuII--2.8, 2.1; NotI--5.0; HIndIII--4.5, 0.3, 0.25; PstI--4.3, 0.65, 0.25. A PstI digest excises the insert as two fragments (bp): 593, 230. The smaller fragment contains the 5' untranslated region. The insert contains the following restriction sites (bp from the 5' end): HindIII--569, 809; NcoI--450; NotI--372; PstI--224; PvuII--405; SacI--154. Contains the complete coding sequence (nt 152-679). |
